Clinical Orthopaedics and Related Research ®

A Publication of The Association of Bone and Joint Surgeons ®

Basic Research 171 articles

Articles

Prolotherapy Induces an Inflammatory Response in Human Tenocytes In Vitro

Emmanuel C. Ekwueme PhD, Mahir Mohiuddin BS, Jazmin A. Yarborough, P. Gunnar Brolinson DO, Denitsa Docheva PhD, Hugo A. M. Fernandes PhD, Joseph W. Freeman PhD

Proliferative therapy, or prolotherapy, is a controversial treatment method for many connective tissue injuries and disorders. It involves the injection of a proliferant, or irritant solution, into the site of injury, which causes small-scale cell death. This therapeutic trauma is theorized to initiate the body’s wound-healing cascade, perhaps leading to tissue repair. The immediate effects of many of these proliferants are poorly characterized, as are the cellular responses to them; here, we sought to evaluate the immediate effects of two common proliferants (dextrose and P2G, a combination of phenol, glucose, and glycerin) on the cellular response of human tenocytes, and begin to explicate the mechanisms with which each proliferant functions.

Combined Administration of ASCs and BMP-12 Promotes an M2 Macrophage Phenotype and Enhances Tendon Healing

Richard H. Gelberman MD, Stephen W. Linderman MPhil, Rohith Jayaram MS, Anna D. Dikina PhD, Shelly Sakiyama-Elbert PhD, Eben Alsberg PhD, Stavros Thomopoulos PhD, Hua Shen PhD

Outcomes after intrasynovial tendon repair are highly variable. An intense inflammatory cascade followed by a delayed healing response can cause adhesion formation and repair-site failure that severely impair the function of repaired digits. No effective remedies exist to fully address these issues. Cell- and growth factor-based therapies have been shown to modulate inflammation and improve cell proliferation and matrix synthesis and therefore are promising treatment approaches for intrasynovial tendon repair.

Does Extracellular DNA Production Vary in Staphylococcal Biofilms Isolated From Infected Implants versus Controls?

Beata Zatorska MSc, Marion Groger PhD, Doris Moser PhD, Magda Diab-Elschahawi MD, MSc, Luigi Segagni Lusignani MD, Elisabeth Presterl MD, MBA

Prosthetic implant infections caused byandare major challenges for early diagnosis and treatment owing to biofilm formation on the implant surface. Extracellular DNA (eDNA) is actively excreted from bacterial cells in biofilms, contributing to biofilm stability, and may offer promise in the detection or treatment of such infections.

Do Mesenchymal Stem Cells Derived From Atypical Lipomatous Tumors Have Greater Differentiation Potency Than Cells From Normal Adipose Tissues?

Hiroyuki Inatani MD, Norio Yamamoto MD, PhD, Katsuhiro Hayashi MD, PhD, Hiroaki Kimura MD, PhD, Akihiko Takeuchi MD, PhD, Shinji Miwa MD, PhD, Takashi Higuchi MD, Kensaku Abe MD, Yuta Taniguchi MD, Satoshi Yamada MD, PhD, Kiyofumi Asai MD, PhD, Takanobu Otsuka MD, PhD, Hiroyuki Tsuchiya MD, PhD

The p53 protein in mesenchymal stem cells (MSCs) regulates differentiation to osteogenic or adipogenic lineage. Because p53 function is depressed in most malignancies, if MSCs in malignancy also have p53 hypofunction, differentiation therapy to osteogenic or adipogenic lineage may be an effective treatment. We therefore wished to begin to explore this idea by evaluating atypical lipomatous tumor/well-differentiated liposarcoma (ALT/WDL) cells, because murine double minute 2 (MDM2) gene amplification, which leads to p53 hypofunction, is found in almost all ALT/WDLs.

Which Clinical and Patient Factors Influence the National Economic Burden of Hospital Readmissions After Total Joint Arthroplasty?

Steven M. Kurtz PhD, Edmund C. Lau MS, Kevin L. Ong PhD, Edward M. Adler MD, Frank R. Kolisek MD, Michael T. Manley FRSA, PhD

The Affordable Care Act of 2010 advanced the economic model of bundled payments for total joint arthroplasty (TJA), in which hospitals will be financially responsible for readmissions, typically at 90 days after surgery. However, little is known about the financial burden of readmissions and what patient, clinical, and hospital factors drive readmission costs.

What Is the Timing of General Health Adverse Events That Occur After Total Joint Arthroplasty?

Daniel D. Bohl MD, MPH, Nathaniel T. Ondeck BS, Bryce A. Basques MD, Brett R. Levine MD, Jonathan N. Grauer MD

Despite extensive research regarding risk factors for adverse events after total joint arthroplasty (TJA), there are few publications describing the timing at which such adverse events occur.

Exposure to Secondhand Smoke Impairs Fracture Healing in Rats

Hildemberg A. R. Santiago PhD, Ariane Zamarioli PhD, Manoel D. Sousa Neto PhD, Jose B. Volpon MD, PhD

Nonsmokers may be affected by environmental tobacco smoke (secondhand smoke), but the effects of such exposure on fracture healing have not been well studied.

What Are the Biomechanical Properties of the Taylor Spatial Frame™?

Daniel J. Henderson FRCS (Orth), Jeremy L. Rushbrook FRCS (Orth), Paul J. Harwood FRCS (Orth), Todd D. Stewart PhD

The Taylor Spatial Frame™ (TSF) is a versatile variant of the traditional Ilizarov circular fixator. Although in widespread use, little comparative data exist to quantify the biomechanical effect of substituting the tried-and-tested Ilizarov construct for the TSF hexapod system.

Defective Bone Repair in C57Bl6 Mice With Acute Systemic Inflammation

D. A. Behrends MD, D. Hui BSc, C. Gao MD, PhD, A. Awlia MD, Y. Al-Saran MD, A. Li MD, J. E. Henderson PhD, P. A. Martineau MD

Bone repair is initiated with a local inflammatory response to injury. The presence of systemic inflammation impairs bone healing and often leads to malunion, although the underlying mechanisms remain poorly defined. Our research objective was to use a mouse model of cortical bone repair to determine the effect of systemic inflammation on cells in the bone healing microenvironment.

Do Needleless Knots have Similar Strength as the Krackow Suture? An In Vitro Porcine Tendon Study

Chih-Kai Hong MD, Ting-Hsuan Kuo, Ming-Long Yeh PhD, I-Ming Jou MD, PhD, Cheng-Li Lin MD, Wei-Ren Su MD, MSc

Numerous needleless techniques for tendon graft fixation that feature several advantages have been reported. However, there are few studies that have compared the holding strength between the needleless techniques (rolling hitch and modified rolling hitch) and traditional suture methods.